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empty control vector pcdna-nc  (Shanghai GenePharma)

 
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    Structured Review

    Shanghai GenePharma empty control vector pcdna-nc
    miR-195-5p suppresses the proliferation, migration and invasion of AMC-HN-8 cells by <t>regulating</t> <t>E2F3</t> expression. (A) E2F3 overexpression efficiency was detected by western blot analysis. (B) miR-195-p mimic and <t>pcDNA-E2F3</t> were co-transfected into AMC-HN-8 cells and cell proliferation was detected by Cell Counting Kit-8 assays. (C and D) Cell migration in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by wound-healing assays. Magnification, x100. (E and F) Cell invasion in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by Transwell assays. Magnification, x100. (G) Western blot analysis and (H) RT-qPCR assay were respectively used to measure the protein and mRNA levels of the epithelial-mesenchymal transition-associated proteins (E-cadherin, vimentin, N-cadherin and snail) and MMP-2 and -9. ** P<0.01; *** P<0.001. miR, microRNA; E2F3, E2F transcription factor 3; miR, microRNA; NC, negative control; MMP, matrix metalloprotease.
    Empty Control Vector Pcdna Nc, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+control+vector+pcdna-nc/pcdna+empty+vector/pmc08355640-42-6-13
    Average 90 stars, based on 1 article reviews
    empty control vector pcdna-nc - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "MicroRNA-195-5p suppresses the proliferation, migration, invasion and epithelial-mesenchymal transition of laryngeal cancer cells in vitro by targeting E2F3"

    Article Title: MicroRNA-195-5p suppresses the proliferation, migration, invasion and epithelial-mesenchymal transition of laryngeal cancer cells in vitro by targeting E2F3

    Journal: Experimental and Therapeutic Medicine

    doi: 10.3892/etm.2021.10512

    miR-195-5p suppresses the proliferation, migration and invasion of AMC-HN-8 cells by regulating E2F3 expression. (A) E2F3 overexpression efficiency was detected by western blot analysis. (B) miR-195-p mimic and pcDNA-E2F3 were co-transfected into AMC-HN-8 cells and cell proliferation was detected by Cell Counting Kit-8 assays. (C and D) Cell migration in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by wound-healing assays. Magnification, x100. (E and F) Cell invasion in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by Transwell assays. Magnification, x100. (G) Western blot analysis and (H) RT-qPCR assay were respectively used to measure the protein and mRNA levels of the epithelial-mesenchymal transition-associated proteins (E-cadherin, vimentin, N-cadherin and snail) and MMP-2 and -9. ** P<0.01; *** P<0.001. miR, microRNA; E2F3, E2F transcription factor 3; miR, microRNA; NC, negative control; MMP, matrix metalloprotease.
    Figure Legend Snippet: miR-195-5p suppresses the proliferation, migration and invasion of AMC-HN-8 cells by regulating E2F3 expression. (A) E2F3 overexpression efficiency was detected by western blot analysis. (B) miR-195-p mimic and pcDNA-E2F3 were co-transfected into AMC-HN-8 cells and cell proliferation was detected by Cell Counting Kit-8 assays. (C and D) Cell migration in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by wound-healing assays. Magnification, x100. (E and F) Cell invasion in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by Transwell assays. Magnification, x100. (G) Western blot analysis and (H) RT-qPCR assay were respectively used to measure the protein and mRNA levels of the epithelial-mesenchymal transition-associated proteins (E-cadherin, vimentin, N-cadherin and snail) and MMP-2 and -9. ** P<0.01; *** P<0.001. miR, microRNA; E2F3, E2F transcription factor 3; miR, microRNA; NC, negative control; MMP, matrix metalloprotease.

    Techniques Used: Migration, Expressing, Over Expression, Western Blot, Transfection, Cell Counting, Quantitative RT-PCR, Negative Control

    Related Articles

    Over Expression:

    Article Title: MicroRNA-195-5p suppresses the proliferation, migration, invasion and epithelial-mesenchymal transition of laryngeal cancer cells in vitro by targeting E2F3
    Article Snippet: The E2F3 overexpression vector pcDNA-E2F3 and empty control vector pcDNA-NC were constructed by Shanghai GenePharma Co., Ltd.

    Plasmid Preparation:

    Article Title: MicroRNA-195-5p suppresses the proliferation, migration, invasion and epithelial-mesenchymal transition of laryngeal cancer cells in vitro by targeting E2F3
    Article Snippet: The E2F3 overexpression vector pcDNA-E2F3 and empty control vector pcDNA-NC were constructed by Shanghai GenePharma Co., Ltd.

    Construct:

    Article Title: MicroRNA-195-5p suppresses the proliferation, migration, invasion and epithelial-mesenchymal transition of laryngeal cancer cells in vitro by targeting E2F3
    Article Snippet: The E2F3 overexpression vector pcDNA-E2F3 and empty control vector pcDNA-NC were constructed by Shanghai GenePharma Co., Ltd.

    Migration:

    Article Title: MicroRNA-195-5p suppresses the proliferation, migration, invasion and epithelial-mesenchymal transition of laryngeal cancer cells in vitro by targeting E2F3
    Article Snippet: The E2F3 overexpression vector pcDNA-E2F3 and empty control vector pcDNA-NC were constructed by Shanghai GenePharma Co., Ltd.

    Expressing:

    Article Title: MicroRNA-195-5p suppresses the proliferation, migration, invasion and epithelial-mesenchymal transition of laryngeal cancer cells in vitro by targeting E2F3
    Article Snippet: The E2F3 overexpression vector pcDNA-E2F3 and empty control vector pcDNA-NC were constructed by Shanghai GenePharma Co., Ltd.

    Western Blot:

    Article Title: MicroRNA-195-5p suppresses the proliferation, migration, invasion and epithelial-mesenchymal transition of laryngeal cancer cells in vitro by targeting E2F3
    Article Snippet: The E2F3 overexpression vector pcDNA-E2F3 and empty control vector pcDNA-NC were constructed by Shanghai GenePharma Co., Ltd.

    Transfection:

    Article Title: MicroRNA-195-5p suppresses the proliferation, migration, invasion and epithelial-mesenchymal transition of laryngeal cancer cells in vitro by targeting E2F3
    Article Snippet: The E2F3 overexpression vector pcDNA-E2F3 and empty control vector pcDNA-NC were constructed by Shanghai GenePharma Co., Ltd.

    Cell Counting:

    Article Title: MicroRNA-195-5p suppresses the proliferation, migration, invasion and epithelial-mesenchymal transition of laryngeal cancer cells in vitro by targeting E2F3
    Article Snippet: The E2F3 overexpression vector pcDNA-E2F3 and empty control vector pcDNA-NC were constructed by Shanghai GenePharma Co., Ltd.

    Quantitative RT-PCR:

    Article Title: MicroRNA-195-5p suppresses the proliferation, migration, invasion and epithelial-mesenchymal transition of laryngeal cancer cells in vitro by targeting E2F3
    Article Snippet: The E2F3 overexpression vector pcDNA-E2F3 and empty control vector pcDNA-NC were constructed by Shanghai GenePharma Co., Ltd.

    Negative Control:

    Article Title: MicroRNA-195-5p suppresses the proliferation, migration, invasion and epithelial-mesenchymal transition of laryngeal cancer cells in vitro by targeting E2F3
    Article Snippet: The E2F3 overexpression vector pcDNA-E2F3 and empty control vector pcDNA-NC were constructed by Shanghai GenePharma Co., Ltd.



    Similar Products

    empty control vector pcdna-nc  (Shanghai GenePharma)
    90
    Shanghai GenePharma empty control vector pcdna-nc
    miR-195-5p suppresses the proliferation, migration and invasion of AMC-HN-8 cells by <t>regulating</t> <t>E2F3</t> expression. (A) E2F3 overexpression efficiency was detected by western blot analysis. (B) miR-195-p mimic and <t>pcDNA-E2F3</t> were co-transfected into AMC-HN-8 cells and cell proliferation was detected by Cell Counting Kit-8 assays. (C and D) Cell migration in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by wound-healing assays. Magnification, x100. (E and F) Cell invasion in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by Transwell assays. Magnification, x100. (G) Western blot analysis and (H) RT-qPCR assay were respectively used to measure the protein and mRNA levels of the epithelial-mesenchymal transition-associated proteins (E-cadherin, vimentin, N-cadherin and snail) and MMP-2 and -9. ** P<0.01; *** P<0.001. miR, microRNA; E2F3, E2F transcription factor 3; miR, microRNA; NC, negative control; MMP, matrix metalloprotease.
    Empty Control Vector Pcdna Nc, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/empty+control+vector+pcdna-nc/pcdna+empty+vector/pmc08355640-42-6-13
    Average 90 stars, based on 1 article reviews
    empty control vector pcdna-nc - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    miR-195-5p suppresses the proliferation, migration and invasion of AMC-HN-8 cells by regulating E2F3 expression. (A) E2F3 overexpression efficiency was detected by western blot analysis. (B) miR-195-p mimic and pcDNA-E2F3 were co-transfected into AMC-HN-8 cells and cell proliferation was detected by Cell Counting Kit-8 assays. (C and D) Cell migration in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by wound-healing assays. Magnification, x100. (E and F) Cell invasion in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by Transwell assays. Magnification, x100. (G) Western blot analysis and (H) RT-qPCR assay were respectively used to measure the protein and mRNA levels of the epithelial-mesenchymal transition-associated proteins (E-cadherin, vimentin, N-cadherin and snail) and MMP-2 and -9. ** P<0.01; *** P<0.001. miR, microRNA; E2F3, E2F transcription factor 3; miR, microRNA; NC, negative control; MMP, matrix metalloprotease.

    Journal: Experimental and Therapeutic Medicine

    Article Title: MicroRNA-195-5p suppresses the proliferation, migration, invasion and epithelial-mesenchymal transition of laryngeal cancer cells in vitro by targeting E2F3

    doi: 10.3892/etm.2021.10512

    Figure Lengend Snippet: miR-195-5p suppresses the proliferation, migration and invasion of AMC-HN-8 cells by regulating E2F3 expression. (A) E2F3 overexpression efficiency was detected by western blot analysis. (B) miR-195-p mimic and pcDNA-E2F3 were co-transfected into AMC-HN-8 cells and cell proliferation was detected by Cell Counting Kit-8 assays. (C and D) Cell migration in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by wound-healing assays. Magnification, x100. (E and F) Cell invasion in each group following transfection with miR-195-5p and pcDNA-E2F3 was detected by Transwell assays. Magnification, x100. (G) Western blot analysis and (H) RT-qPCR assay were respectively used to measure the protein and mRNA levels of the epithelial-mesenchymal transition-associated proteins (E-cadherin, vimentin, N-cadherin and snail) and MMP-2 and -9. ** P<0.01; *** P<0.001. miR, microRNA; E2F3, E2F transcription factor 3; miR, microRNA; NC, negative control; MMP, matrix metalloprotease.

    Article Snippet: The E2F3 overexpression vector pcDNA-E2F3 and empty control vector pcDNA-NC were constructed by Shanghai GenePharma Co., Ltd.

    Techniques: Migration, Expressing, Over Expression, Western Blot, Transfection, Cell Counting, Quantitative RT-PCR, Negative Control